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NicoBxl · 04-10-2014, 06:41 AM

why do you use a ttest and not DESeq test (based on negative binomial) ? RNA-Seq data do not follow a normal distribution.

for normalized count, you can use also : normalizedCounts <- counts(dds,normalized=TRUE)

04-10-2014, 06:41 AM	#5
NicoBxl not just another member Location: Belgium Join Date: Aug 2010 Posts: 264	why do you use a ttest and not DESeq test (based on negative binomial) ? RNA-Seq data do not follow a normal distribution. for normalized count, you can use also : normalizedCounts <- counts(dds,normalized=TRUE)