Quote:
Originally Posted by Brian Bushnell
For more advanced statistics, particularly if you have a reference and are evaluating different assembly methodologies, I recommend Quast because it also does alignment to the reference to calculate the number of misassemblies.
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Their (excellent, I love SPAdes and QUAST) group is developing
rnaQUAST, to evaluate transcriptome assemblies. Version 0.1.1 (current version at the time of my message) has a bug, though, reference transcriptome file naming has to strictly follow:
I could not use a reference which had:
Code:
name.middle.extension