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Old 05-13-2015, 08:41 AM   #6
nepossiver
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Location: São Paulo

Join Date: Oct 2012
Posts: 8
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Quote:
Originally Posted by Brian Bushnell View Post
For more advanced statistics, particularly if you have a reference and are evaluating different assembly methodologies, I recommend Quast because it also does alignment to the reference to calculate the number of misassemblies.
Their (excellent, I love SPAdes and QUAST) group is developing rnaQUAST, to evaluate transcriptome assemblies. Version 0.1.1 (current version at the time of my message) has a bug, though, reference transcriptome file naming has to strictly follow:

Code:
name.extension
I could not use a reference which had:

Code:
name.middle.extension

Last edited by nepossiver; 05-13-2015 at 09:06 AM. Reason: added rnaQUAST link.
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