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Old 02-14-2017, 06:22 AM   #4
mrfox
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Location: USA

Join Date: Aug 2010
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Quote:
Originally Posted by SylvainL View Post
It will depend on the frame and orientation... You could make a short script extracting the nucleotides or the region (let's say 5 upstream and 5 downstream) and translate it yourself ...
Thank you both. Yes it depends on the ORF and orientation. Technically, we have to concatenate the exon DNA sequences of a transcript and count the position of the mutation and translate the short sequences according to the ORF.

Thanks!
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