SEQanswers - View Single Post - Unexpected 425bp band un Truseq stranded mRNA library prep

nucacidhunter · 04-03-2017, 05:58 PM

I think it is most likely amplicon contamination if you do not have a designated pre- and post-PCR lab. You can also pay attention to see if the contaminated libraries are from particular columns or rows of the plate.

I have seen small extra peak (like GR-11, GR-23) when the TapeStation shakes during loading but most of yours are large peaks and the same size.

04-03-2017, 05:58 PM	#2
nucacidhunter Jafar Jabbari Location: Melbourne Join Date: Jan 2013 Posts: 1,238	I think it is most likely amplicon contamination if you do not have a designated pre- and post-PCR lab. You can also pay attention to see if the contaminated libraries are from particular columns or rows of the plate. I have seen small extra peak (like GR-11, GR-23) when the TapeStation shakes during loading but most of yours are large peaks and the same size.