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Old 02-09-2018, 01:12 PM   #1
Junior Member
Location: Madison, WI, USA

Join Date: Jan 2011
Posts: 6
Question long-range technologies for genome scaffolding

Hi all,

I am working on a mammalian genome project. We have an assembly from PacBio reads with N50 = 6.2 Mb. We would like to generate some long-range data that could be used for scaffolding. I am aware of several options, including Nanopore, Hi-C (with Dovetail or Phase Genomics), optical maps (Bionano), and synthetic long reads (10X). Can you share your experiences with these technologies? We need help choosing which one(s) to use.


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