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Old 02-09-2018, 01:12 PM   #1
ybukhman
Junior Member
 
Location: Madison, WI, USA

Join Date: Jan 2011
Posts: 6
Question long-range technologies for genome scaffolding

Hi all,

I am working on a mammalian genome project. We have an assembly from PacBio reads with N50 = 6.2 Mb. We would like to generate some long-range data that could be used for scaffolding. I am aware of several options, including Nanopore, Hi-C (with Dovetail or Phase Genomics), optical maps (Bionano), and synthetic long reads (10X). Can you share your experiences with these technologies? We need help choosing which one(s) to use.

Thanks.

Yury
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