SEQanswers - View Single Post - PCR duplicates increase when excess of beads

tdm · 03-30-2011, 12:55 PM

Hi PMiguel,
I have the data you ask for:
Type of library: SureSelect All Human Exon Capture (50Mb)
Sequencing: Fragment (50bp), barcoded samples, 6 samples/slide
How many cycles of PCR: As low as possible. Ususally 10 cycles in pre and post capture PCR. But in that case, we had to do 12 cycles for both pre and post capture PCR.
How many beads/panel: ~280,000 beads/panel

Thank you
Thibault

03-30-2011, 12:55 PM	#3
tdm Member Location: Montreal Join Date: Sep 2010 Posts: 19	Hi PMiguel, I have the data you ask for: Type of library: SureSelect All Human Exon Capture (50Mb) Sequencing: Fragment (50bp), barcoded samples, 6 samples/slide How many cycles of PCR: As low as possible. Ususally 10 cycles in pre and post capture PCR. But in that case, we had to do 12 cycles for both pre and post capture PCR. How many beads/panel: ~280,000 beads/panel Thank you Thibault