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Old 03-31-2011, 08:15 AM   #8
niceday
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Location: cambridge

Join Date: Apr 2010
Posts: 68
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Can I also point to the amount of PCR cycles?
We have been discussing the same problem yesterday.
12 cycles after library production, then size selection then enrichment. This is already producing duplicates.
After enrichment there are usually another 8 to 12 cycles?

We are looking at going into enrichment without PCR and size selection. Not sure if we need to order our own adapters as I believe the agilent adapters for SOLiD enrichment are truncated.

This is how we do our illumina enrichments and we don't get the same duplication problem.

Today we tried 6 cycles of PCR after enrichment giving us a total of 18 overall. We will check the sequence data as it comes off.
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