Hi niceday
Quote:
Originally Posted by niceday
We are looking at going into enrichment without PCR and size selection. Not sure if we need to order our own adapters as I believe the agilent adapters for SOLiD enrichment are truncated.
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With SOLID protocol, it is not possible to go into enrichment without PCR because adapters are truncated and we need the nick translation step to attach correctly the adpaters.
thank you for your help.
Thibault