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Old 03-31-2011, 09:48 AM   #11
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Location: Purdue University, West Lafayette, Indiana

Join Date: Aug 2008
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Technically, you could do a single cycle of PCR to "step-out" your adapters to their full length. That can result in no PCR duplication. Personally, I would do at least 3 cycles. And would only go that low with trepidation. Lots of issues that don't matter at ng/ul concentrations do matter at pg/ul. (DNA binding levels of plastics, for instance.)

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