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Old 06-06-2011, 02:31 PM   #6
ZAAB
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Location: Boston

Join Date: Apr 2011
Posts: 18
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Nah...SYBR or EtBr were both in the gel prior to running it.

15-30minute ligations...OK. thanks. And yes, I agree that some degree of ligation occurred since there's an increase in the amount of correct size fragment.

Its the TruSEQ Illumina prep kit. Following it verbatim other than the Minelute (vs Ampure) because I determined that the insert size was below the threshold of Ampure...nice waste of cash buying the ampure.

Maybe the DNA size is big enough now, but don't think I'll waste my time extracting DNA from the Ampure washes.

Thanks Guys.
Z
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