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Old 09-15-2011, 12:47 PM   #5
james hadfield
Cambridge, UK
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Location: Cambridge, UK

Join Date: Feb 2008
Posts: 221

you could add a 4bp random sequence in your barcode read or at the 5'end of your oligo for ligation. This way you can see if a read is a duplicate of PCR. You should not see the same random sequence, unless PCR has amplified it so.
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