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Old 06-23-2009, 05:31 AM   #3
Junior Member
Location: South Africa

Join Date: Oct 2008
Posts: 5

thanks for your insight James,

Library and qPCR product samples run on agarose side-by-side indicate the size difference as well.

Unfortunately we do not have a phiX library to PCR as control. However, most of the libraries we used in the qPCR have been sequenced on the GA already.

One question
: Did you run your PCR products from your qPCR assay on a gel and get the same length as the library? Even when using a taqman assay, with PCR primers annealing to the ends of your library amplicons, you'd expect to amplify products of the same length as the library. If so, what primers have you been using? My primers are designed to anneal to the termini of the adapters.

Any suggestions about how to go about the taqman assay would welcome.
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