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Old 10-17-2017, 06:24 PM   #1
setramis
Junior Member
 
Location: Perú

Join Date: Oct 2017
Posts: 3
Default Illumina MiSeq High cluster density, but low %PF problem

Hello everyone.

Please, I need a help with my MiSeq genome sequencing run. I am sequencing M. tuberculosis genomes using Nextera XT and a 500 cycles v2 cartridge/flow cell. I always obtained aproximately 950-1050 K/mm2 and a passing filter more than 85%; However, in my last run I obtained 1063k/mm2 (i think it is ok), BUT JUST 50,3% %PF... sincerely, I dont have any idea what happened. I know with overclustering there will be a lower %PF, but in think is not my case. I s the first time i have this issue.

Thank in advance for your answers.
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