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Old 10-17-2013, 11:43 AM   #2
Devon Ryan
Location: Freiburg, Germany

Join Date: Jul 2011
Posts: 3,480

The Lister/Ecker 2009 dataset is directional. When in doubt, just align the first 100,000 or so reads as non-directional and then look at the alignment statistics. If it's not actually non-directional, then the complementary strands will have very few reads aligned to them.
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