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Old 10-18-2013, 08:50 AM   #5
pmiguel
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Location: Purdue University, West Lafayette, Indiana

Join Date: Aug 2008
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The problem is if you are using some other method to look for 5-methylation of cytosines, it may not be possible. For example, if you use Me-DIP, then there is no conversion, you just see the unconverted sequence and assay for peaks.

It also kind of hurts my head to think that you can take a TruSeq DNA library prior to amplification, perform a methyl-cytosine immunoprecipitation on it. (This presumes you used non-methylated Y-adapters!) Then amplify and sequence it. This library will be directional! Because of the Y-adapters.

Not sure it gains you anything, but there you are...
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Phillip
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