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Old 10-18-2013, 08:04 AM   #9
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Location: Purdue University, West Lafayette, Indiana

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Originally Posted by dpryan View Post
Yeah, the whole directional/non-directional thing is specific to bisulfite treatment. When we start using nanopores that can directly read a base as methylated then this stuff will quickly become a historical footnote.
And yet, the MeDIP libraries I describe above are, in fact, directional. But I don't think there is a way to detect that informatically after the fact.

But yes, we are talking the about technology, rather than science. (Neal Stephenson has a hilarious section of his book, Anathem, that highlights the difference.)

BTW, my guess is direct detection of methylated bases will be commercially available from Pac Bio before they are from Oxford Nanopore.

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