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Old 12-06-2013, 07:55 AM   #4
FiReaNG3L
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Location: Paris

Join Date: Apr 2012
Posts: 17
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My version is a custom one I implemented before their Methods paper got published - I never tested their solution.

It's not more difficult, it's just more labor intensive. It's basically a Chip + a library prep on the same day. If you follow their protocol to the letter, its 30 washes of 5 min, plus the enzymatic steps. The proper analysis also requires more work if you want to use the extra information the technique gives you.

Depending on your final goal, it could not be worth the extra work / costs or (as in my case) it could be essential. From your first post I would say if you don't really care about the fine resolution, you're better off with Chip-seq.
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