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Old 01-27-2014, 08:12 AM   #14
Location: Europe

Join Date: Sep 2012
Posts: 39

The antibody should be sound.

We've been wondering if it is more a case of the exonuclease failing to stop at the crosslinking site, while stopping at various points before. This could be due to misfolding and be stricly dependant on the TF being analysed.

This exonuclease-stop hypothesis seems to be supported by the high variability in peak width I'm observing (which I verified via cross-correlation approaches to look at the start-stop distance - these fail to show anything apart from the phantom peak, suggesting there is no consensus start-stop distance). The fact that our TF binds in several configurations (monomeric/dimeric) does not help.
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