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Old 01-28-2014, 04:34 AM   #15
TonyBrooks
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Location: London

Join Date: Jun 2009
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Quote:
Originally Posted by james hadfield View Post
Forgot to direct you to this: Development of an Illumina-based ChIP-exonuclease method provides insight into FoxA1-DNA binding properties.

http://genomebiology.com/2013/14/12/R147/abstract
Thanks for that James. We're about to look into ChIP-Exo and designed our own primers, but we may just use these instead. Am I right in thinking that there is a T missing from their P7-exo adapters though? They are doing blunt-end ligation, but the Illumina R1 and R2 sequencing primers end in T due to the dA-tail ligation generally employed.
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