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Old 04-23-2017, 04:12 PM   #4
nucacidhunter
Jafar Jabbari
 
Location: Melbourne

Join Date: Jan 2013
Posts: 1,225
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Quote:
Originally Posted by Carcharodon View Post
When I did this before, a couple of my libraries did amplify beautifully, so I know it works. The failures have made me a bit gun-shy.
I wonder by "failure" you mean that a digested-ligated DNA PCR was successful without sized-selection and failed after size-selection. If that is the case I would column purify Pippin eluate or dilute it for PCR or use different DNA polymerase because Pippin buffer composition can inhibit some polymerases activity.
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