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Old 04-23-2017, 08:42 PM   #6
nucacidhunter
Jafar Jabbari
 
Location: Melbourne

Join Date: Jan 2013
Posts: 1,225
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You can use 5% (or any other quantity depending on your workflow) of digested-ligated DNA to do a PCR and resolve amplicons. It should show the size region with poor amplification so you can avoid those regions during size-selection.
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