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Old 08-28-2017, 10:39 AM   #2
Brian Bushnell
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Location: Walnut Creek, CA

Join Date: Jan 2014
Posts: 2,707

5' is most likely biological, a known artifact of fragmentation site bias and should be ignored. Nextera libraries in particular demonstrate this bias very strongly but it does not seem to have much impact downstream, and trimming it won't accomplish anything.
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