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Old 09-11-2017, 10:04 AM   #6
Brian Bushnell
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Location: Walnut Creek, CA

Join Date: Jan 2014
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Quote:
Originally Posted by DeondeJager View Post
For the sample with overrepresented kmers at the 5' end (sample2 in fastqc plots above = sample B98_289 in histograms below) there is a slight decrease in the match rate for the 5' and 3' ends of the reads, compared to the middle of the reads. However, this is not different from a sample that had no overrepresented kmers anywhere (sample B98_579). Therefore, it seems that the overrepresented kmers are genomic and not synthetic. Would you agree?
Yes, it sounds like it's probably fine to me... no trimming needed.
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