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Old 07-15-2019, 07:18 AM   #1
Cistron
Junior Member
 
Location: UK

Join Date: Feb 2013
Posts: 9
Question Removal of <110bp primer-dimers without beads?

Hi all,

I was wondering whether anyone has come across a column-based cleanup kit, which would remove large primers (EMP 16S design) and primer-dimers of around 100bp. Or alternatively, protocols to "abuse" silica columns to behave accordingly.

I'm running a course with students, so I am pretty sure the budget won't stretch for beads and magnetic plates. Unfortunately, given my number of students and time-constraint, I cannot always do the right thing and get the PCR optimised properly - I'm happy it works most of the time .

I've also come across a sample recently (raw milk), where all my troubleshooting wouldn't get rid of the pesky primer-dimers. So I can appreciate that this would happen to one of my flock.

Thanks!
Mike
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