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Old 12-09-2011, 08:44 AM   #3
delinquentme
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Location: pittsburgh

Join Date: Jul 2011
Posts: 12
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so lets break this down into steps

1) Nextera. DNA
This kit ? It mentions Illumina .. does it work for the Ion PGM?

2) Fragmentation to ligation in one quick and easy step
This sounds like its part of the kit? or a machine operation?

3) quick low cycle PCR
Did some googling and this doesn't seem like its run-of-the-mill PCR ... So do I need a special machine?

4) validation
I think this is the quantitation step ( the DNA / liquid ratio )?

BTW ! thanks for digging back into these articles!

Last edited by delinquentme; 12-09-2011 at 08:46 AM. Reason: added thanks!
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