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Old 12-13-2011, 05:38 AM   #2
Scoob
Junior Member
 
Location: London

Join Date: Feb 2010
Posts: 4
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For me the Clontech SMARTer kit worked beautifully. Used 100pg total RNA and generated nice Bioanalyser profiles. Also titrated down from 50 to 1 cell and got good products with all of them. You do have to optimise the cycle number, of course, and at the single cell level (which is way below the spec of the kit) there is some inevitable variation, but its pretty impressive.
Tried the Nugen kit and didnt really get on with it, but maybe that was just me.
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