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Old 12-09-2013, 07:08 AM   #5
Michael Love
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Location: Boston

Join Date: Jul 2013
Posts: 333
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hi John,

The VST helps to stabilize the variance over the mean, insofar as this can be captured by the parametric curve of dispersion over mean. You might also try the rlog transformation, which sometimes performs qualitatively better than the VST (for example, if the size factors vary a lot across samples).
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