View Single Post
Old 12-21-2013, 08:01 AM   #5
Junior Member
Location: Boston Ma

Join Date: Mar 2012
Posts: 8

I see. To clarify for others, during forward read, the flow cell has only ssDNA, and the Read 1 primer can only bind to the Read 1 site. The other potential binding site is only present in a reverse complement form. After finishing the Read 1 phase, the flow cell is switched to containing the reverse complement of the original ssDNA, cleaving the original ssDNA and washing it away. The Read 2 primer can now bind. So, in principle, the Read 1 an Read 2 primers could be identical, and we would still get directional sequencing.
Thank you.
Phage434 is offline   Reply With Quote