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Old 12-21-2013, 08:01 AM   #5
Phage434
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Location: Boston Ma

Join Date: Mar 2012
Posts: 8
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I see. To clarify for others, during forward read, the flow cell has only ssDNA, and the Read 1 primer can only bind to the Read 1 site. The other potential binding site is only present in a reverse complement form. After finishing the Read 1 phase, the flow cell is switched to containing the reverse complement of the original ssDNA, cleaving the original ssDNA and washing it away. The Read 2 primer can now bind. So, in principle, the Read 1 an Read 2 primers could be identical, and we would still get directional sequencing.
Thank you.
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