I would like to generate small RNA/miRNA libraries from small quantities of total RNA. The Illumina TruSeq Small RNA Library Prep kit requires an input of 1ug of total RNA for input.
Has anyone tried to find the lowest input required -- while still maintaining the appropriate library complexity ? Ideally, I'd like to start with 1-50ng of total RNA.
Is there an alternate low input small RNA protocol that anyone's aware of ?
Any help would be greatly appreciated !
Thanks,
Michael
Has anyone tried to find the lowest input required -- while still maintaining the appropriate library complexity ? Ideally, I'd like to start with 1-50ng of total RNA.
Is there an alternate low input small RNA protocol that anyone's aware of ?
Any help would be greatly appreciated !
Thanks,
Michael
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