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Old 11-13-2013, 04:28 PM   #1
thomasblomquist
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Location: Ohio

Join Date: Jul 2012
Posts: 68
Default Targeted quantitative RNA-sequencing with interlibrary concordance and reduced cost

http://www.plosone.org/article/info%...l.pone.0079120

Hello SEQanswers Forum. Long time follower, occasional thread responder. I have been in the qPCR world for some years now. One need in the RNA-seq world I've noticed is the need for the assays to be, 1) targeted, 2) quantitative, 3) reproducible in quantitation, and 4) at a drastically reduced cost.

The work in the study presented above represents our approach to make targeted RNA-sequencing highly quantitative, reproducible, at a drastically reduced need for sequencing depth for large dynamic ranges of templates.

Hope you all enjoy. Any questions as to how this study can be applied to your research needs, I would be more than happy to address.

-Tom Blomquist
University of Toledo
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