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Old 11-26-2013, 12:21 PM   #2
KristenC
Junior Member
 
Location: New Zealand

Join Date: Nov 2013
Posts: 7
Default Interesting!

Hi Tom,

I really enjoyed reading your paper! You touch on a couple of very important issues present in targeted RNA-sequencing.
I am currently working on a targeted RNA-sequencing assay, and am exploring possibilities for using reference genes to normalize the counts. Since your assay does not even need reference genes, I was wondering if you at some point looked into it and maybe dismissed it for certain reasons? Could you comment on why you moved away from possible adaptations of the more traditional RNA-seq approaches?

Kind regards,

Kristen
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