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Old 01-09-2014, 11:19 AM   #1
DMD
Junior Member
 
Location: Boston

Join Date: Jan 2014
Posts: 2
Red face Need help with a GenBank submission

Hello everyone,

New to the forum, but I have a question which I hope someone can help me with. I am trying to submit to GenBank Roche 454 16S sequences but is having a small problem. We used the pipeline QIIME for our alignment etc. However, when you submit to GenBank, you need at least the sequence and the organism name. The QIIME pipeline is a bit different. It generates a sequence file and then a phylogeny file which is matched by an identifier. My problem is that I have at least 122,000 sequences. It is nearly impossible for me to perform this manually. This is probably easiest done via a script of some sort (unfortunately I have no script writing ability).

Does anyone know of any open sourced software or of any other programs I could use to resolve this problem. We have already submitted the paper so submitting the sequences is a high priority (I didn't expect it to be this difficult).
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