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Old 12-19-2014, 07:05 PM   #6
nucacidhunter
Jafar Jabbari
 
Location: Melbourne

Join Date: Jan 2013
Posts: 1,238
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Quote:
Originally Posted by JenBarb View Post
I am involved with a metagenomics study where I have 200-250bp sequence reads from Next Gen Sequencing derived from different regions of the 16s gene, i.e. 6 different primers (primer sequences are unknown as they are from a commercial kit and the company informed us that they are proprietary). Jen
If you are trying to find out the primer sequences used for amplifying 16S region in a particular kit, the easiest way would be preparing NGS libraries form amplicons by using a different NGS platform. For instance, if the kit is designed for platform ITxx, you can amplify the regions and then after clean up use ampliconas as input into ILxx library prep. Sequencing would be expected to start from primers.
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