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Old 03-06-2015, 09:01 AM   #1
slls9969
Junior Member
 
Location: California

Join Date: Mar 2015
Posts: 1
Default ion proton. Reads length much shorter than library size?

Hi,

Recently I'm doing RNAseq on ion proton. I used ion total-RNA seq v2 kit to make the libraries and checked the size distribution on fragment analyzer. The average size of all libraries was 170-190bp. However, when I sequenced them on ion proton, the average read length is only around 79bp. Does anyone have any suggestions how I can increase the read length? The template kit and sequence kit I'm using is Ion PI Template OT2 200 kit v3 and Ion PI sequencing 200 kit v3. I'm a new user of Ion proton, so any suggestion is appreciated.
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