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Old 12-28-2015, 09:23 PM   #1
Junior Member
Location: Oman

Join Date: Nov 2014
Posts: 9
Default Rerun of cycle sequencing

Hi All

Recently I have run a cycle sequencing reaction, however the run was stopped due to a fatal error in the thermocycler. it stopped at cycle 8 of 25 cycles for approximately an hour till I realized the error message .then, I decided to rerun the cycle sequencing using different machine immediately.

from your experience , is the reaction is going to work if I sequence it. Should I stop and repeat the whole reaction again?

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