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Old 02-19-2016, 02:29 AM   #1
Location: Aarhus, Denmark

Join Date: Mar 2008
Posts: 57
Default Degraded RNA from bacteria biofilms following Ribo-Zero

We have applied Ribo-Zero to two sets of RNA isolated from bacteria. Set A was isolated from bacteria grown as biofilm and set B was from bacteria in culture. The total RNA is fine from both sets (RIN 9-10) but following Ribo-Zero the RNA from set A (biofilm) is degraded while the RNA from set B looks fine. As all Ribo-Zero reactions was conducted in the same batch, I am confident that "something" is present in the RNA from biofilms which make the RNA degrade during Ribo-Zero treatment.
Appreciate suggestions to troubleshoot.
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