View Single Post
Old 10-18-2016, 02:29 AM   #2
nucacidhunter
Jafar Jabbari
 
Location: Melbourne

Join Date: Jan 2013
Posts: 1,238
Default

Less wasteful approach is contacting tech support but if you want to optimise it yourself then set up one reaction and take aside 2ul samples for running on Bioanalyzer at 8, 10 and 12 minutes. I also wonder if 100-150 bp is insert size after library prep or just after fragmentation. Salts in fragmentation buffer can speed up fragments migration giving a false shorter than actual size. Also fragment size will depend on input and 15 min is for input RNA with high RIN.

Last edited by nucacidhunter; 10-18-2016 at 02:32 AM.
nucacidhunter is offline   Reply With Quote