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Old 10-18-2017, 01:05 PM   #3
provirus
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Location: Denmark

Join Date: Oct 2017
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It would also be informative with bioanalyzer profiles showing: a) input (the 200 ng) alone and no library prep, b) library prep with input but no adapters, c) library prep with adapter but no input, d)-f) library preps with 10x fold dilutions (1x, 0.1x, 0.01x) of the adapter, and finally g) your dna together with a "standard" commercial adapter using standard protocol.

Even though you are sure that everything "is fine" (mass and that it is covarized correctly etc) many things might have gone wrong that you did not think of - the controls above might point to steps that are not optimal.
Good luck.
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