View Single Post
Old 03-16-2018, 07:30 AM   #3
Senior Member
Location: CT

Join Date: Apr 2015
Posts: 243

The error that halted the first run was (can't remember exact wording) can't find focus without boosting signal beyond threshold. Looking at the thumbnails, the actual focus was fine but there weren't many clusters and it seems the software just gave up after base 6 of the first index.

We do the template line bleach wash between each run. And both machines pass the volume check. Not sure how the volume check works though, is there a time component that would tell me if the pressure gets as high as it's supposed to within the flowcell?

I'm doing a phiX run on the machine where the library failed using a flow cell from the same lot as the ones that look funky. I'll see what that looks like
Microbial ecologist, running a sequencing core. I have lots of strong opinions on how to survey communities, pretty sure some are even correct.
thermophile is offline   Reply With Quote