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Old 03-19-2018, 06:33 AM   #5
Senior Member
Location: CT

Join Date: Apr 2015
Posts: 243

The phiX run failed. Engineer is coming tomorrow. It's possible that it was flow cell related, I used the last of my flowcells from that lot on the phiX run.

I ran another primarily amplicon library on the machine that didn't fail (second machine in my OP). It looks beautiful, ~750k, 75% PF, 80% >Q30. So I'm left with something weird about the library + machine issues OR flow cell issues. I'm going to try mixing the problem library with nextera genomes that I've sequenced before to bump up the base diversity rather than more phiX since they have indexes.
Microbial ecologist, running a sequencing core. I have lots of strong opinions on how to survey communities, pretty sure some are even correct.
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