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Old 04-20-2020, 08:51 AM   #6
GenoMax
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If we assume all 10000 cells survived. You want a minimum 50000 reads per cell so that is 500M reads * 2 conditions = 1 Billion clusters/single-end reads total. Sequencing length if fixed based on the kit used so you should just worry about reads than basepairs.

According to 10x this will give you 30-50% saturation. If you need more saturation then you will need to sequence more. This assumes that libraries work/sequence well.

Last edited by GenoMax; 04-20-2020 at 12:18 PM.
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