View Single Post
Old 04-23-2020, 09:30 AM   #9
invu
Junior Member
 
Location: Boston, MA, USA

Join Date: Apr 2020
Posts: 5
Default

Quote:
Originally Posted by cement_head View Post
I'd have to agree with GenoMax; super-important to have a consultation with the sequencing center about the library composition and ask them what they recommend. You probably should have had 10% PhiX spike-in added. HiSeq are terrible at dynamic calibration - MiSeqs are better (to a point).
I see. Next time I will consider PhiX spike-in. Thanks, cement_head!
invu is offline   Reply With Quote