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Old 08-03-2020, 08:15 AM   #1
ETBA
Junior Member
 
Location: UK

Join Date: Sep 2018
Posts: 4
Default Sequencing Human/Mouse ATAC libraries together?

Hi all,

I am a novice to this and currently preparing mouse and human ATAC libraries. All samples are on different barcodes and we want to compare these samples, so we think it's better to sequence (100 bp, paired end, HiSeq-X-Ten) everything together to get similar depth across all and reduce batch effects.

I'm unsure if this is a problem for the sequencer or for the analysis too? After read-trimming and FastQC check, when I go to align the samples with BWA-MEM or Bowtie2 for example, could I run one analysis and select for aligned human and mouse reads or do two separate runs targeted to 1 species to separate them out?

Any help / suggestions are much appreciated.

Thanks, Eric
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