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Old 03-21-2014, 10:23 AM   #1
dtm2451
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Location: San Francisco, Ca

Join Date: Mar 2013
Posts: 9
Default Extra peaks in a bioanalyzer trace for library made from ePCR amplicon

Hello,

I am working on a deep sequencing protocol for a PCR amplicon (~650bp) using the TruSeq DNA PCR-Free Sample Preparation Kit and I am seeing extra peaks in my final bioanalyzer traces that concern me because I don't know what they might come from.

Peaks on the trace:
+Small peak at size of original insert
+Medium-sized peak that I think might correspond to insert+1adapter
+Large peak that I think might correspond to insert+2 adapters
-mini peak to the right of the "insert" peak
-mini peak to the right of the "insert+1adapter" peak
-mini peak to the right of the "insert+2adapters" peak

Does anyone know what the last three peaks might be?

I am attaching both the TapeStation on the initial PCR material and the bioanalyzer on the final library.

Thanks!
Dan
Attached Files
File Type: pdf Electropherograms.pdf (392.2 KB, 228 views)
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