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Old 06-09-2014, 02:13 AM   #2
nucacidhunter
Jafar Jabbari
 
Location: Melbourne

Join Date: Jan 2013
Posts: 1,238
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1) For bacterial genome it would not make much difference in result. The consideration would be cost and availability of DNA input amount.

2) qPCR based quantification (not using normaliser beads) will increase chance of even number of read output for samples.

3) KAPA qPCR certainly is better than Qubit. Qubit only measures mass of DNA not cluster ready portion of library.

4) This question is a bit unclear to me. Using high molecular weight input DNA (without sonication) will give more consistent results. Sonication is not in the protocol and I do not see any reason for sonication.

Last edited by nucacidhunter; 06-09-2014 at 02:30 AM.
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