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Old 09-15-2015, 02:28 PM   #2
jteeee2
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Location: Illinois

Join Date: Oct 2014
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I have also seen considerably low yields post Ribo-Zero for bacterial samples. Have you tried simply proceeding into library prep with the material you have? The success of this strategy might depend on the kit manufacturer you are using, but I can tell you that I have recently proceeded into the Kapa Biosystems RNA-Seq kit using a low amount of RNA (I can't tell you the exact mass because I couldn't detect it on Qubit with 1 uL input) and generated successful libraries. It might be worth a shot with one of your samples. Best of luck!
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