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Old 09-16-2015, 10:59 AM   #3
ScienceGrrl
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Location: Indianapolis, IN

Join Date: Jul 2015
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I had the opposite problem in my previous position of too much sample at the end b/c of failure to remove rRNA. I tested my depletion using Illumina's suggestion of running qPCR assay using GAPDH and 18S to see the amount of material before and after depletion. If your non-rRNA species are low in the pre depletion, you may be getting all you can.
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