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Old 09-16-2015, 03:36 PM   #4
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Location: Bioo Scientific, Austin, TX, USA

Join Date: Jun 2012
Posts: 119

Have you tried inputting more total RNA into the ribo-depletion, or do you have limited amounts?

Also, 1-2 ng should be fine for many RNA-Seq library prep protocols, so you may want to look into an alternative protocol; there are lots of companies making RNA-Seq kits these days. Feel free to PM me for more information in Bioo Scientific's offerings. As a disclaimer, I work for Bioo Scientific.
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