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Old 02-25-2016, 01:31 PM   #1
jlove
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Location: Boston, MA

Join Date: Apr 2011
Posts: 49
Default Assay to test for ligation efficiency?

Hi,
Has anyone got a method for assessing TruSeq ligation efficiency, pre-PCR? We use the KAPA Illumina library quant kit for final library quantification, and I can certainly try using this, but I am wondering if anyone knows about actually using the results to assess the efficiency? I'm not sure how to evaluate the results to determine next steps.
Thanks in advance!
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