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Old 02-26-2016, 09:48 AM   #2
jdk787
josh kinman
 
Location: Austin

Join Date: Apr 2014
Posts: 71
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Quote:
Originally Posted by jlove View Post
Hi,
Has anyone got a method for assessing TruSeq ligation efficiency, pre-PCR? We use the KAPA Illumina library quant kit for final library quantification, and I can certainly try using this, but I am wondering if anyone knows about actually using the results to assess the efficiency? I'm not sure how to evaluate the results to determine next steps.
Thanks in advance!
You could try ligating, then quantifying your sample with Qubit to determine the total amount of DNA present.
Then quantify the same sample with the KAPA quantification kit which will only measure the amount of ligated material in your sample.
Ligation efficiency can then be determined by comparing the Qubit number to the Kapa number.
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